Glycosaminoglycan production by bovine aortic endothelial cells cultured in sulfate-depleted medium.
نویسندگان
چکیده
منابع مشابه
Glycosaminoglycan Production by Bovine Aortic Endothelial
Bovine aortic endothelial cells were cultured in medium containing [3H]glucosamine and concentrations of [“S]sulfate ranging from 0.01 to 0.31 mM. While the amount of [‘H]hexosamine incorporated into chondroitin sulfate and heparan sulfate was constant, decreasing concentrations of sulfate resulted in lower [‘“SIsulfate incorporation. Sulfate concentrations greater than 0.1 1 mM were required ...
متن کاملProstacyclin production by perturbed bovine aortic endothelial cells in culture.
This study reports that endotoxin (Escherichia coli serotype 026:B6) and 12-O-tetradecanoyl-phorbol-13-acetate stimulate cultured bovine aortic endothelial cells to generate prostacyclin. The prostacyclin concentration of the culture medium was measured indirectly by radioimmunoassay for 6-keto-PGF1 alpha. The amount of prostacyclin generated depended on the concentration of endotoxin or phorbo...
متن کاملHeparan Sulfate in Bovine Aortic and Pulmonary Artery Endothelial Cells
Newly synthesized heparan sulfates purified from the cell layer of bovine aortic endothelial cells (BAECs) and main pulmonary artery endothelial cells (BPAECs) cultured under either normoxic (21% oxygen) or hypoxic (3% oxygen) conditions were characterized by size, charge, and capacity to bind to antithrombin III. Incorporation of radiolabeled sulfate into cell layer-associated heparan sulfate ...
متن کاملRenin - Angiotensin Components in Cultured Bovine Aortic Endothelial Cells
Cultured bovine aortic endothelial cells were examined for renin activity by biochemical, immunological, and immunohistochemical techniques. When cell sonicates were incubated with renin substrate, linear generation of angiotensin I was observed (1.12 ± 0.2 ng angiotensin I/10 cells per hr). The effect of pH on this activity was similar to that of bovine renal renin, and renin antibodies inhibi...
متن کاملEthanolamine metabolism in cultured bovine aortic endothelial cells.
The role of extracellular ethanolamine in phospholipid synthesis was examined in cultured bovine aortic endothelial cells. Serine and ethanolamine were both readily accumulated by these cells and incorporated into phospholipid. Exposing cells to extracellular ethanolamine for 4-6 weeks had no effect on cell growth, yet increased the phosphatidylethanolamine content of these cells by 31% as comp...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1986
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)67626-4